Two subsets of primers were successfully used to amplify two portions of the N315 chromosome. A set of primers was generated from the genome sequence of S.aureus N315, employed here as a reference strain. GenoFrag was developed for the analysis of Staphylococcus aureus genome plasticity by whole genome amplification in ~10 kb‐long fragments. Posted on 4 4 Categories PCR / Primer Design Tags BatchPrimer3, High Throughput, PCR, Primer Design, Sequencing, Web Application RJPrimers 1.0 – Transposable Element Junction based PCR Primer Design You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson OD.īMC Bioinformatics. A batch input of large number of sequences and a tab-delimited result output greatly facilitates rapid primer design and ordering process.ĭepartment of Plant Sciences, University of CaliforniaīatchPrimer3: a high throughput web application for PCR and sequencing primer design. BatchPrimer3 allows users to design several types of primers including generic primers, hybridization oligos, SSR primers together with SSR detection, and SNP genotyping primers (including single-base extension primers, allele-specific primers, and tetra-primers for tetra-primer ARMS PCR), as well as DNA sequencing primers. BatchPrimer3 is a comprehensive web primer design program using Primer3 core program as a major primer design engine to design different types of PCR primers and sequencing primers in a high-through manner.
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